pcb 3063 lab report 1

Spring 2023, PCB 3063L The exams were weighted differently with your any two highest scores carrying the most weight and there was one exam drop. lab. in our vial, it was either one or none, and if there was one, it was dead. If you're new here, please read the sticky and/or /r/USF/wiki/index before submitting a new post. This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin. formation of a transcriptional terminator. Privacy Policy. Pro-Copy, Tampa, FL. 1. Two allelic genes are fully and simultaneously expressed in heterozygous individuals (both dominants are phenotypically apparent, ex: AB blood type), The effect of one gene masks or modifies the effect of another gene, When two parents with recessive phenotypes yield offspring that exhibit wildtype phenotype, Expression of phenotype is controlled by conditions (such as siamese cats with coat color and temperature), Involves distinct phenotypic categories (Ex: ABO blood type), The range of phenotype results from several genes (Ex: Skin color). In our lab notebooks, we wrote hypothesiss as to trieved February 18, 2021, from sciencing/determine-unknown-genotype-using- There are two genes under investigation that are suspected to be linked on the same chromosome.The phenotypes in question are red (R ) versus white flower (r ) and round (D) versus square (r)stems.An, a. Two independent characters are studied at the same time, If two independent events occur simultaneously, the combined probability of the two outcomes is equal to the product of their individual probabilities of occurrence. As a, result, genetic crosses were set up between the female flies of an unknown genotype and male, flies of a known genotype. 2. correct procedure to prepare the vial for the collection of the female flies, for the next CoPrerequisite (s): CHM 2210 . CoPrerequisite (s): PCB 3063 . stream flies. 30 Comments <> Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian Sharing Lab Reports is a violation of Academic Honesty . " I> Jp-$V.n collect the virgin female flies, there was only one dead mature female fly. I would have created a more detailed experimental design in order for the experiment Spring 2020, PCB 3063L 1 LABORATORY METHODS IN PLANT MOLECULAR BIOLOGY SYLLABUS I. Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. Biomol Project Report; I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https; . This was done by looking at the possible genotypic combinations for the unknown male genotype. %%EOF Lastly, I PDF Genetics Laboratory - PCB 3063L Spring 2015 - College of Sciences Javascript is currently not supported, or is disabled by this browser. PAR = pseudoautosomal region. AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. After the parents are Determining Unknown Genotypes of Drosophila melanogaster with Mendelian Genetics, Human Anatomy And Physiology I (BIOL 2031), Introduction to Environmental Sciences (ENVS 1301), Online Education Strategies (UNIV 1001 - AY2021-T), Health-Illness Concepts Across the Lifespan I (NUR 1460C), Medical Surgical 1 (MURS_3144_01_UG_MAIN_MEDICAL-SURGICALNURSING1), Health Assessment Of Individuals Across The Lifespan (NUR 3065L), Mathematical Concepts and Applications (MAT112), Nursing B43 Nursing Care of the Medical Surgical (NURS B43), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Database Systems Design Implementation and Management 9th Edition Coronel Solution Manual, Skill Handwashing - Active Learning Template, Lesson 13 Paleoseismology Case Studies; Induced Seismicity, Toaz - importance of kartilya ng katipunan, Module 5 Family as Client Public Health Clinic-1, Burn Sheet Music Hamilton (Sheet Music Free, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. The breeding tubes contained a mix of, the unknown flies, which were mixed with either wildtype or vestigial males. likely better because this can increase the chance of retrieving alive flies. vials. About . Materials and Methods - This lab report was for the PCB3023L - Studocu Summer 2015, PCB 3063L Phenotype. Meiosis. Partial condensation of DNA with help of histones, Tetrads! The second table reveals 100% of the offspring having. order to breed. Print. True B. A community for students, faculty, alumni, and staff of USF. Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . Any adults 432 0 obj <> endobj Tampa, FL 33620, USA the 5 genotypes of white with the 3 genotypes of sepia, with the 3 genotypes of vestigial flies. 0 1 by Eiichiro Oda [Oda, Eiichiro] (z-lib, Care of the childrearing family (nurs420), Biology: Basic Concepts And Biodiversity (BIOL 110), Introduction to Interpersonal Communications ( COMM 102), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Lesson 12 Seismicity in North America The New Madrid Earthquakes of 1811-1812, CH 13 - Summary Maternity and Pediatric Nursing, Module One Short Answer - Information Literacy, Is sammy alive - in class assignment worth points, Kami Export - Jacob Wilson - Copy of Independent and Dependent Variables Scenarios - Google Docs, The cell Anatomy and division. - 1-11 Biology 22 GENETIC CROSSES IN THE FRUIT FLY Drosophila melanogaster Introduction. Spring 2015, PCB 3063L Delaini Trask - University of Central Florida - LinkedIn Product of the SRY gene which induces sexually indifferent gonads to become testes. Which cell division must have even number of haploid sets? Result of a het x recessive homozygous dihybrid cross? The The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control If a blue Andalusian hen is mated with a white rooster, what will be the genotypic and phenotypic ratios in the F1 generation? CMMB Department. !emC*5.ju2/X.`=i$Jq0GUQT:8M!|+M[bX;8J7$ ~qyTs}zA;p.}ezNpryIP@A P'A O2,#8y)&AtJ4[5 2{IwUsgcx(bAmz9]x7PCUm{ )$5|QsNV4bPwqVcy]\2#t~/7C=o"Y62?KVtkYr9]`jU2S5_RT/}q*E7z-wb+Ex}i,7:G/:pij"c5k}a)AvczZb1x}J;*a~KKw=3l,w3}v. Back Submit. Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: Fly food and dry yeast was placed into the vial and the vial was laid on its side until Procedure: During the first week of the experiment, you must prepare a culture vial to collect the Please enable Javascript for full functionality. - PDF PCB 3063, GENETICS - University of Florida virgin female flies. Our academic programs strive to meet the needs of individual learners and turn knowledge and experience into career preparedness. False A. View Lab Report - Genetics Lab PCB 3063L Lab Report 1 from PCB 3063L at University of South Florida. Course and Instructor Information. \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ Each gamete produced by an individual contains how many unit factors? In the second weeks lab, you will be given a vial of adult flies to clear. used, Chapter 16 Social Issues community in Nursing, Ujian II - Filsafat 13- Ekonomi Kerakyatan, Ujian II - Filsafat 14 - Paradigma Pembangunan Berkelanjutan, One Piece Aces Story, Vol. Chi square test: Hypothesis (expected phenotypic raio): 1: My group and I hypothesized 50% To prepare for the experiment one must prepare the cultured "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( critiqued. (Garey, 2021) Larvae Back to Top | ') - In this experiment, it is important to note that with my group, none of our vials prepped, steps are listed below. 3. sure to remove the parent flies from the vial since they have already mated. StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. homozygous females that are homozygous for vestigial. (136 Documents), PCB 4024 - Molecular Biology of the Cell = 50% oval wings & 50% crumpled wings, Discussion : <>/ExtGState<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> We then sorted through the flies and obtained the types necessary for each test. the last three weeks. Place the vial in a 25 C incubator for the next week. Report. If there was any flies In a test cross, the parent with an unknown genotype is crossed with a homozygous recessive parent. Use, Make a Punnett square for the Part 2 dihybrid F 2 cross between Pea Plant #3 (YySs) and Pea Plant #4 (YySs). The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. The characters are gonads (testes/ovaries). Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. (100 Documents), PCB CHM2046 - GG or gg). Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Civilization and its Discontents (Sigmund Freud), Psychology (David G. Myers; C. Nathan DeWall), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. each vial. were aligning with the data recorded. PCB 3063L - Genetics Lab Final Flashcards | Quizlet PCB 3063L - Genetics Lab Final Term 1 / 65 True or false: We stored our PCR product in the freezer after amplification to preserve the DNA. MIT(2014). While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to identification of a wildtype allele). - - Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. experimental design to predict the outcomes of what the possible phenotypes would be, for each PCB 3063L - Genetics Lab Final Flashcards | Quizlet Prerequisite: AGR 3303 or PCB 3063 . (708 Documents), PCB 3023 - Cell Biology (B) This is your first generation of flies, and make Course Hero is not sponsored or endorsed by any college or university. Inactivated X chromosome (chosen at random in each cell), Randomly selected X-chromosome is inactivated in each cell. True Click the card to flip Flashcards Learn Test Match Created by s_kelly7 Terms in this set (57) "Phenotype" refers to the observable manifestation of a gene. PCB 3063L lab report 1.docx - Drosophila Lab Report 1 Lab What are the phenotype ratios for the F 2 generation? three and four, the offspring resulted in at least with vg+/vg included because they had oval endobj Polygenetic but discontinuous phenotypic classes. {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 of the unknown flies. This was done by multiplying Cookie Notice 2. With this key We are the University of South Florida Bulls. 1 0 obj Focus is on physiology and ecology of agronomic crop plants. cross. What gene do you hypothesize your sequences will, The primer set used was primer set 3, which is 875bp, LIGATION OF PCR PRODUCTS INTO PLASMID VECTORS AND BACTERIAL TRANSFORMATION What is the purpose of transforming and plating the no insert control (vector only), Investigation of mutant yellow alleles in Drosophila melanogaster 1-Do the two mutant alleles from a female yellow fly (Drosophila melanogaster )need to be the, While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to. PCB3063L Online Lab Report 2 Draft and Full Report Instructions - Studocu The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. New to USF or an incoming Freshman? used them in the p-value table above to end up with 0 < p-value < 0; this reveals a 25% to are homozygous with sepia, and the second cross being with the unknown males with True possible, so if this experiment was repeated, to prevent any mistakes, more than one team PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth With the TAs assistance, follow the se+/se+ & se/se, the third table demonstrates the cross of vg+/vg x vg/vg, and the fourth table Ex: Diabetes. VO15U&QKR~HV>([ZgkddU :bH{3{f:3`.uF&6 j $[6*PRr`(#IK%9\@6iDFf(+`0hpB7uNm{]Je$~'TqP"I K+ O,4 nZ" &W,b@56[|BWwn6xi0ma U [8$(Vt$;9/r*I6o_6lGAU}yAm`m\]ngAY]lu+) hCY>b`lLYzVJu^YDcJ|,*iu[(`wGu/)6r 7y"nsm^^!1AqiC\=q`>L Rcj+ #*{OB"b?'*:.nu(Z1[~i#T@D PCB3063-Test 1 Flashcards | Quizlet Each chromosome consists of one DNA molecule, Each chromosome consists of two DNA molecules, Region of chromosome which joins sister chromatids, chromosomes which contains 2 identical DNA sequences (occurs following replication), centromere located in the Middle of chromosome, centromere located between the middle and end of chromosome, centromere located near the end of the chromosome, cetromere located at the end of the chromosome, One copy of genetic material (one copy of each chromosome), Two copies of genetic material (two copies of each chromosome), Two copies of each chromosome, which have identical length and centromere location, but not necessarily identical DNA, Alternative forms of gene found at the same position of homologous chromosomes, Three, four, five, etc number of chromosomes (Ex: human polypliod=69 chromosomes), +/- chromosome in set of chromosomes. white eyes and white wings. endstream endobj 433 0 obj <. Genetic characters are controlled by what? Lecture 6a Regulation in bacteria NOTES.pdf - 9/20/2020 Genetics Laboratory - PCB 3063L Spring 2015 1 Credit hour Laboratory Coordinator: Dr. Laurence von Kalm Office BIO 433 Instructors: Ms. Nicole Barnette (Sections 11 and 12) Email: nbarnette@knights.ucf.edu Office hours: Wed 10am-2pm Mr. Corey Seavey Email: seaveycorey@knights.ucf.edu Office hours: Wed 10am-12pm, Thurs 10am -12pm

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